Staff at its Seattle headquarters staged a protest last year as the company tightened the full remote work allowance that was put in place during the pandemic.
Amazon subsequently fired the organiser of the protest, prompting claims of unfair retaliation, a dispute that has been taken up with labour officials.
In his message on Monday, Mr Jassy said he was worried that Amazon - which has long prided itself on preserving the intensity of a start-up while growing to become a tech giant - was seeing its corporate culture diluted by flexible work and too many bureaucratic layers.